(Determination of Hyoscyamine and Scopolamine in Datura stramonim L. by High Performance Liquid Chromatography.
Gyong-Sam Do, Min-Kyo Shin, Ho-Joon Song, Choong-Sin Seon, In-Kyung Son and Seung-Hwa Baek.
Director of public Health, Institute for Health and Environment of Dae-Jean City, 102-1. Tan bang dong, Dae-jean, Korea.
Dept of Oriental medicine, College of Oriental Medicine, Won Kwang University, 570-749, Iri, Korea.
Dept of Chemistry, College of Natural Science. Won Kwang University, 570-749, Iri, Korea.
Abstract-A new method for quantitative determination of Hyoscyamine and Scopolamine in Datura stramonium L. by High Performance Liquid Chromatography (HPLC) was established. A reversed-phase system with a μBondapak C18 column using methanol in
0.2%
aqueous phosphoric acid(40:60) with PIC A as a mobile phase was developed.
Hyoscyamine and Scopolamine was detected at 254nm and the analysis was successfully carried out within 20min.
Keywords-Datura stramonium L. Hyoscyamine. Scopolamine. HPLC. Solanaceae. Tropane Alkaloid.
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